activity of catalase.

Aim: To investigate on the activity of catalase. Introduction Catalase, an enzyme put up in many different tissues, catalyses the breakdown of total heat henry peroxide into piss and atomic number 8. 2H₂O₂ → 2H₂O + O₂ atomic way out 1 peroxide is a toxic substance that tail assembly be formed during aerobic respiration and catalase removes this product. The activity of catalase butt joint be measured by finding the rate of oxygen release from hydrogen peroxide. Minced murphy provides a suitable radical of catalase and the pH is varied in this experiment using citric acid-sodium orthophosphate buffer dissolvers at pH values of 4.4, 5.2, 6.5, 7.5 and 9. assumption: As the value of pH increases, the numerate of oxygen increases up to a point where the tot decreases. This is because the active site is denatured and the 3 dimensional anatomy is altered. Apparatus: Citric acid-sodium phosphate buffer solutions of pH 4.4, 5.2, 6. 5, 7.5 and 9 Hydrogen peroxide solution Potato 10ml and 100ml measuring piston chamber Stopwatch bear and clamp Water bucket pH paper, pH step and pH probe connected to info logger.

Cork woodborer Large test tube Rubber stopper and economy tube Risk sound judgement/safety precautions Risk assessment sentry go precautions Citric acid may be irritant simulate gloves, lab coat, and safety look Oxidising hydrogen peroxide is corrosive go to pieces gloves, lab coat, and safety goggles Fragile glassware eg measuring cylinderPlace glassware in the tenderness of the table Variables: Independent variant: pH level certified variant: average volume of oxygen produced Control variable: Same size of po! tato, same amount of hydrogen peroxide. prejudicial and non destructive testing of material: Destructive testing: hydrogen peroxide, pH, potato Non destructive testing: pH meter does not alter the solution, test tube, measuring cylinder Method: 1.Obtained prick as shown below 2.Used a cork borer, cut cylinder of...If you want to get a full essay, order it on our website: OrderEssay.net

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